RB1CC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 10043-2-AP

Synonyms

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

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Validation Data Gallery

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10043-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10043-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10043-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10043-2-AP

  Immunofluorescent analysis of Hela cells, using RB1CC1 antibody 10043-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse brain tissue, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Product Information

10043-2-AP targets RB1CC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: RB1-inducible coiled-coil 1
• Calculated Molecular Weight: 1594 aa, 183 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC017556
• Gene Symbol: RB1CC1
• Gene ID (NCBI): 9821
• RRID: AB_2253571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation[PMID:15968549 ]. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation [PMID: 19258318]. It is probably involved in the tumorigenesis of breast cancer[PMID: 14533007]. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene[PMID: 21602932]. The immunogen of this antibody is C-terminal 154aa of RB1CC1.

Publications

Species	Application	Title
mouse	WB	Cell; Noncanonical autophagy promotes the visual cycle.; Authors - Kim Ji-Young JY ; KD Validated
human	WB	Nat Immunol; Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.; Authors - Michal P Wandel ; KD Validated
human	WB	Nat Cell Biol; mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6.; Authors - Nazio Francesca F
human	IF	Nat Cell Biol; ATM functions at the peroxisome to induce pexophagy in response to ROS.; Authors - Jiangwei Zhang
mouse	WB	Genes Dev; p62/SQSTM1 synergizes with autophagy for tumor growth in vivo.; Authors - Huijun Wei
human, mouse	WB, IF	Nat Struct Mol Biol; Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and -independent autophagy.; Authors - Noor Gammoh

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