RB1 Monoclonal antibody

RB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A2A6

Cat no : 67521-1-Ig

Synonyms

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1

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Validation Data Gallery

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  WB analysis of Jurkat using 67521-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67521-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 67521-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67521-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67521-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 67521-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of TF-1 using 67521-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of Insulinoma using 67521-1-Ig

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 67521-1-Ig (RB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Insulinoma using 67521-1-Ig

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 67521-1-Ig (RB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 67521-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using RB1 antibody (67521-1-Ig, Clone: 1A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of U2OS using 67521-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RB1 antibody (67521-1-Ig, Clone: 1A2A6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, HL-60 cells, K-562 cells, MCF-7 cells, TF-1 cells
• Positive IHC detected in: Insulinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67521-1-Ig targets RB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RB1 fusion protein Ag22578
• Full Name: retinoblastoma 1
• Calculated Molecular Weight: 928 aa, 106 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC039060
• Gene Symbol: RB1
• Gene ID (NCBI): 5925
• RRID: AB_2882741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Publications

Species	Application	Title
human	WB	Cancer Res; CDK4/6 Alters TBK1 Phosphorylation to Inhibit the STING Signaling Pathway in Prostate Cancer; Authors - Wei Li
human	WB	Life (Basel); Investigating the Effects of Chelidonic Acid on Oxidative Stress-Induced Premature Cellular Senescence in Human Skin Fibroblast Cells; Authors - Burcu Turkoglu