RB1 Polyclonal antibody

RB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30373-1-AP

Synonyms

pRb, pp110, p110-RB1 , p110-RB1, p105-Rb

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Validation Data Gallery

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  WB analysis using 30373-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30373-1-AP (RB1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 30373-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30373-1-AP (RB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 30373-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30373-1-AP (RB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 30373-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RB1 antibody (30373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: K-562 cells, HT-1080 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30373-1-AP targets RB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: retinoblastoma 1
• Calculated Molecular Weight: 928 aa, 106 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC039060
• Gene Symbol: RB1
• Gene ID (NCBI): 5925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The RB1 gene, a member of the pocket family with p107 and p130, was the first known tumor suppressor. RB1protein is targeted by the transforming proteins of the DNA tumor viruses like adenoviral E1A and is functionally inactivated in most human tumor cells owing to mutations of either the RB1 gene itself or its upstream regulators (PMID: 22787429).