RASGRP2 Polyclonal antibody

RASGRP2 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30189-1-AP

Synonyms

GRP2, F25B3.3 kinase-like protein, Cdc25-like protein, CDC25L, CalDAG-GEFI

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Validation Data Gallery

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  WB analysis using 30189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30189-1-AP (RASGRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 30189-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human RASGRP2 (30189-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells, Raji cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

30189-1-AP targets RASGRP2 in WB, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASGRP2 fusion protein Ag32762
• Full Name: RAS guanyl releasing protein 2 (calcium and DAG-regulated)
• Calculated Molecular Weight: 69kd
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: NM_153819
• Gene Symbol: RASGRP2
• Gene ID (NCBI): 10235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RASGRP2, also named as CDC25L and MCG7, belongs to the RASGRP family. It functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. RASGRP2 may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. RASGRO2 functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. It may function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. The antibody recognizes the C-term of RASGRP2.

Publications

Species	Application	Title
human	WB,CoIP	Biochim Biophys Acta Mol Cell Res; NEDD4L-mediated RASGRP2 suppresses high-glucose and oxLDL-induced vascular endothelial cell dysfunctions by activating Rap1 and R-Ras; Authors - Guozhu Chen