RASGRF1 Monoclonal antibody, PBS Only

RASGRF1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4D4

Cat no : 67010-1-PBS

Synonyms

CDC25, CDC25L, GNRP, GRF1, GRF55, H GRF55, PP13187, Ras GRF1, RAS-GRF, RASGRF1

---

Validation Data Gallery

• 

  WB analysis of pig cerebellum using 67010-1-Ig (same clone as 67010-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67010-1-Ig (same clone as 67010-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 67010-1-Ig (same clone as 67010-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67010-1-Ig (same clone as 67010-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

---

Product Information

67010-1-PBS targets RASGRF1 in WB, Indirect ELISA applications and shows reactivity with rat, pig samples.

• Tested Reactivity: rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RASGRF1 fusion protein Ag6937
• Full Name: Ras protein-specific guanine nucleotide-releasing factor 1
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC040275
• Gene Symbol: RASGRF1
• Gene ID (NCBI): 5923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.