RASGAP Polyclonal antibody

RASGAP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12935-1-AP


CM AVM, CMAVM, GAP, GTPase activating protein, p120GAP, p120RASGAP, PKWS, Ras p21 protein activator, RASA, RASA1, RASGAP

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12935-1-AP targets RASGAP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASGAP fusion protein Ag3906
Full Name RAS p21 protein activator (GTPase activating protein) 1
Calculated Molecular Weight 1047 aa, 116 kDa
Observed Molecular Weight 120 kDa, 95 kDa
GenBank Accession NumberBC033015
Gene Symbol RASA1
Gene ID (NCBI) 5921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for RASGAP antibody 12935-1-APDownload protocol
IHC protocol for RASGAP antibody 12935-1-APDownload protocol
IF protocol for RASGAP antibody 12935-1-APDownload protocol
IP protocol for RASGAP antibody 12935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.

Authors - Wang Junfeng J

Int J Clin Exp Pathol

Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.

Authors - Xiaojuan Ye