RASGAP Polyclonal antibody

RASGAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12935-1-AP

Synonyms

RASA1, Ras p21 protein activator, Ras GTPase-activating protein 1, p120RASGAP, p120GAP

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Validation Data Gallery

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  WB analysis using 12935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12935-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12935-1-AP

  IP result of anti-RASGAP (IP:12935-1-AP, 4ug; Detection:12935-1-AP 1:4000) with mouse testis tissue lysate 1120 ug.

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  IHC staining of human testis using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12935-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RASGAP antibody (12935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12935-1-AP targets RASGAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASGAP fusion protein Ag3906
• Full Name: RAS p21 protein activator (GTPase activating protein) 1
• Calculated Molecular Weight: 1047 aa, 116 kDa
• Observed Molecular Weight: 120 kDa, 95 kDa
• GenBank Accession Number: BC033015
• Gene Symbol: RASA1
• Gene ID (NCBI): 5921
• RRID: AB_2175906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Biochem Biophys Res Commun; miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.; Authors - Wang Junfeng J
human	WB	Int J Clin Exp Pathol; Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.; Authors - Xiaojuan Ye