RARS2 Polyclonal antibody

RARS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16079-1-AP

Synonyms

Arginine tRNA ligase, Arginyl tRNA synthetase like, ArgRS, DALRD2, PCH6, PRO1992, RARS2, RARSL

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Validation Data Gallery

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  WB analysis using 16079-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16079-1-AP (RARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, human placenta tissue, mouse placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16079-1-AP targets RARS2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RARS2 fusion protein Ag9074
• Full Name: arginyl-tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 578 aa, 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC010420
• Gene Symbol: RARS2
• Gene ID (NCBI): 57038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RARS2 encodes the mitochondrial arginyl transfer RNA (tRNA) synthetase, an enzyme which belongs to the group of mitochondrial aminoacyl tRNA synthetases. These nuclear encoded mitochondrial proteins play a key role in mitochondrial protein translation by catalyzing the attachment of amino acids to their cognate tRNA molecules . Variants in RARS2 gene are associated with pontocerebellar hypoplasia type 6 (PCH6; MIM 611523), a rare mitochondrial encephalopathy with phenotype characterized by early onset seizures, profound developmental delay, and progressive pontocerebellar atrophy.(PMID: 27769281, PMID: 33209735)