RARS Monoclonal antibody

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  WB analysis of HeLa using 66815-1-Ig

  WB result of RARS antibody (66815-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells.

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  WB analysis using 66815-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66815-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66815-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66815-1-Ig (RARS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66815-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RARS antibody (66815-1-Ig, Clone: 1C8G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

RARS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66815-1-Ig

Clone No.1C8G4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DALRD1, ArgRS, Arginyl-tRNA synthetase, arginyl tRNA synthetase, Arginine--tRNA ligase, cytoplasmic

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, MCF-7 cells, A431 cells, HEK-293 cells, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66815-1-Ig targets RARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RARS fusion protein Ag26399
• Full Name: arginyl-tRNA synthetase
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC000528
• Gene Symbol: RARS
• Gene ID (NCBI): 5917
• RRID: AB_2882158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54136
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.