RARB Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RARB Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14013-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14013-1-AP (same clone as 14013-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14013-1-AP (RARB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse bladder using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of rat bladder using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14013-1-AP (same clone as 14013-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RARB antibody (14013-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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Product Information

14013-1-PBS targets RARB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RARB fusion protein Ag5119
• Full Name: retinoic acid receptor, beta
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 37-51 kDa
• GenBank Accession Number: BC060794
• Gene Symbol: RARB
• Gene ID (NCBI): 5915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Retinoic acid receptors (RARs) are important mediators of retinoid signaling in morphogenesis, development, and cell differentiation. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PMID:12554770). They can bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, they act mainly as activators of gene expression due to weak binding to corepressors. RARB is one isotypes of RARs (PMID:12834868).