RAPH1 Polyclonal antibody

RAPH1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25750-1-AP

Synonyms

ALS2CR18, ALS2CR9, KIAA1681, Lamellipodin, LPD, PREL 2, PREL2, Proline rich EVH1 ligand 2, Protein RMO1, RalGDS/AF 6, RAPH1, RMO1

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Validation Data Gallery

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  WB analysis of SKOV-3 using 25750-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25750-1-AP (RAPH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 25750-1-AP

  Immunofluorescent analysis of HepG2 cells using 25750-1-AP (RAPH1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SKOV-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25750-1-AP targets RAPH1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAPH1 fusion protein Ag22705
• Full Name: Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
• Calculated Molecular Weight: 1250 aa, 135 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC156922
• Gene Symbol: RAPH1
• Gene ID (NCBI): 65059
• RRID: AB_2880223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAPH1 encodes the lamellipodin protein (Lpd), which binds to Ena and VASPs to assist in actin cytoskeleton assembly; in this way, the gene plays an important role in cellular motility and lamelipodial protrusion. This protein has 9 isoforms producing different molecular weights of 135 kDa, 67 kDa, 70 kDa, 73 kDa.