EPAC1 Polyclonal antibody

EPAC1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 12572-1-AP

Synonyms

RAPGEF3, RAPGEF3, EPAC1, EPAC 1, EPAC, CGEF1

---

Validation Data Gallery

• 

  WB analysis of CHO using 12572-1-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 12572-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12572-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 12572-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 12572-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: CHO cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12572-1-AP targets EPAC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

• Tested Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPAC1 fusion protein Ag3240
• Full Name: Rap guanine nucleotide exchange factor (GEF) 3
• Calculated Molecular Weight: 881 aa, 99 kDa
• Observed Molecular Weight: 99-104 kDa
• GenBank Accession Number: BC017728
• Gene Symbol: EPAC1/RAPGEF3
• Gene ID (NCBI): 10411
• RRID: AB_11232407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPAC1 functions as a guanine nucleotide exchange factor (GEF), activating small GTPases like Rap1 and Rap2, leading to diverse cellular response. Epac proteins are cAMP sensors that regulate several pivotal cellular processes, including calcium handling, cell proliferation, cell survival, cell differentiation, cell polarization, cell adhesion events, gene transcription, secretion, ion transport, and neuronal signaling. It is a potential target for therapeutic intervention, such as metabolic disorders, cardiovascular diseases (PMID: 20007405, PMID: 20421979). EPAC1 also enhances brown fat growth and beige adipogenesis (PMID: 38195707).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tongbin (Verified Customer) (08-25-2020)

This EPAC1 antibody specifically detects EPAC1 in human cell lines.

• Applications: Western Blot
• Cell Tissue Type: HEK293T