RAP1B Polyclonal antibody

• Featured Product
• KD/KO Validated

RAP1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 10840-1-AP

Synonyms

GTP binding protein smg p21B, K REV, RAL1B, RAP1, RAP1B, Ras related protein Rap 1b

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Validation Data Gallery

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  WB analysis of A549 using 10840-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:300. .

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  WB analysis using 10840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis using 10840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10840-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10840-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10840-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10840-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 10840-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10840-1-AP (RAP1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 10840-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10840-1-AP (RAP1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, A549 cells, C6 cells, A431 cells, human brain tissue, HepG2 cells, mouse kidney tissue, NIH/3T3 cells
• Positive IHC detected in: human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10840-1-AP targets RAP1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAP1B fusion protein Ag1282
• Full Name: RAP1B, member of RAS oncogene family
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC000176
• Gene Symbol: RAP1B
• Gene ID (NCBI): 5908
• RRID: AB_2253539
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.

Publications

Species	Application	Title
human	WB	Mol Cell; Macrophage-Associated PGK1 Phosphorylation Promotes Aerobic Glycolysis and Tumorigenesis.; Authors - Yajuan Zhang
human	WB	Oncotarget; LncRNA MT1JP functions as a tumor suppressor by interacting with TIAR to modulate the p53 pathway.; Authors - Lihui Liu
human	WB	Int J Mol Sci; Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B.; Authors - Sonja Hinz
chicken	WB,IP	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
human	WB	Am J Cancer Res; Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B; Authors - Jiao Liu
human	WB,IF	Viruses; ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells; Authors - Beibei Zhang