RAP1A Recombinant antibody, PBS Only

RAP1A Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240438G5

Cat no : 83708-1-PBS

Synonyms

Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2

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Validation Data Gallery

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  IF Staining of NIH/3T3 using 83708-1-RR (same clone as 83708-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAP1A antibody (83708-1-RR, Clone: 240438G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83708-1-RR (same clone as 83708-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-1-RR, Clone:240438G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83708-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83708-1-RR against Human RAP1A were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83708-1-PBS is RAP1A Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAP1A fusion protein Ag9488
• Full Name: RAP1A, member of RAS oncogene family
• Calculated Molecular Weight: 184 aa, 21 kDa
• GenBank Accession Number: BC014086
• Gene Symbol: RAP1A
• Gene ID (NCBI): 5906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.