RANBP3 Polyclonal antibody

RANBP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27168-1-AP

Synonyms

Ran-binding protein 3, RAN binding protein 3

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Validation Data Gallery

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  WB analysis using 27168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27168-1-AP (RANBP3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 27168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27168-1-AP (RANBP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 27168-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27168-1-AP (RANBP3 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 27168-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RANBP3 antibody (27168-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: mouse testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27168-1-AP targets RANBP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RANBP3 fusion protein Ag26000
• Full Name: RAN binding protein 3
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC004349
• Gene Symbol: RANBP3
• Gene ID (NCBI): 8498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RANBP3 (Ran-binding protein 3) is also named as Rab escort protein 1 (REP-1). RanBP3 is a RanGTP‐binding protein that has been reported to enhance nuclear export of active β‐catenin, independent of its role as a CRM1‐associated nuclear export cofactorl (PMID: 9637251). RanBP3 has been reported to be a crucial downstream molecule of MAPK-ERK and PI3K-AKT signal pathways (PMID: 18331709). RanBP3 is a tumor suppressor in CRC and is associated with patient survival (PMID: 36270974). RanBP3 has been reported as a potential regulator in melanoma biology, hepatocellular carcinoma, prostate cancer, and ovarian cancer, and RanBP3 is highly expressed in CML Cells (PMID: 34504783).