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RALB Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG2a

Reactivity

Capture: Human

Detection: Human, rat, pig, mouse

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 6.25-100 ng/mL

Cat No : MP50129-1

Print datasheet

Synonyms

RALB, Ras related protein Ral B

Validation Data Gallery

  • Cytometric bead array standard curve of MP50129-1, RALB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67094-2-PBS. Detection antibody: 67094-1-PBS. Standard:Ag28600. Range: 6.25-100 ng/mL.

    Cytometric bead array standard curve of MP50129-1

    Cytometric bead array standard curve of MP50129-1, RALB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67094-2-PBS. Detection antibody: 67094-1-PBS. Standard:Ag28600. Range: 6.25-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50129-1 targets RALB in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 6.25-100 ng/mL (Cytometric Bead Array);

Capture antibody: RALB Monoclonal antibody, PBS Only (Capture) 67094-2-PBS (1G7F2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: RALB Monoclonal antibody, PBS Only (Detector) 67094-1-PBS (1D11C11). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard:

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 67094-2-PBS
Tested Reactivity Human
Immunogen RALB fusion protein Ag28600
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.10 x 10-9M
KOff=1.89 x 10-5M
KOn=1.72 x 104M
Full Name v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Synonyms RALB, Ras related protein Ral B
GenBank accession numberBC018163
Gene symbol RALB
UniprotP11234
Gene ID (NCBI) 5899
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 67094-1-PBS
Tested Reactivity Human, rat, pig, mouse
Immunogen RALB fusion protein Ag28600
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Affinity
KD=1.82 x 10-10M
KOff=2.54 x 10-6M
KOn=1.40 x 104M
Full Name v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Synonyms RALB, Ras related protein Ral B
GenBank accession numberBC018163
Gene symbol RALB
UniprotP11234
Gene ID (NCBI) 5899
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.