RAG1 Polyclonal antibody, PBS Only

• 

  WB analysis using 20977-1-AP (same clone as 20977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20977-1-AP (RAG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20977-1-PBS in a different storage buffer formulation.

RAG1 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 20977-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

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Product Information

20977-1-PBS targets RAG1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: recombination activating gene 1
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: NM_000448
• Gene Symbol: RAG1
• Gene ID (NCBI): 5896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15918
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Recombination-activating gene (RAG) proteins include RAG1 and RAG2, which are crucial for Ig and T lymphocyte receptor (TCR) fragment rearrangement and are responsible for DNA recognition and cleavage at specific sequences. Mutations in RAG1 result in the complete or partial loss of recombinant enzyme activity, in unbalanced variable-diversity-joining (V(D)J) recombination, and in the impairment of T and B lymphocyte development at the early stages.