RAF1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RAF1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D6A1

Cat no : 66592-1-PBS

Synonyms

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

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Validation Data Gallery

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  WB analysis using 66592-1-Ig (same clone as 66592-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

  WB result of RAF1 antibody (66592-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DC2.4 using 66592-1-Ig (same clone as 66592-1-PBS)

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAF1 antibody (66592-1-Ig, Clone: 1D6A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

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Product Information

66592-1-PBS targets RAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAF1 fusion protein Ag25423
• Full Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Calculated Molecular Weight: 648 aa, 73 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC018119
• Gene Symbol: RAF1
• Gene ID (NCBI): 5894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.