RAF1 Polyclonal antibody

RAF1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 51140-1-AP

Synonyms

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1



Tested Applications

Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51140-1-AP targets RAF1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-raf-1 murine leukemia viral oncogene homolog 1
Calculated Molecular Weight 648 aa, 73 kDa
GenBank Accession NumberBC018119
Gene Symbol RAF1
Gene ID (NCBI) 5894
RRIDAB_2881251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Protocols

Product Specific Protocols
IHC protocol for RAF1 antibody 51140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
mouse

J Neuroinflammation

Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage.

Authors - Xiaowei Fei
human

Lab Chip

Paper-based microreactor array for rapid screening of cell signaling cascades.

Authors - Chia-Hao Huang
human

Biochem Pharmacol

1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.

Authors - Yuhang Zhou
mouse

Eur J Immunol

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Authors - Satabdi Nandi
human

Food Funct

Dandelion root extract affects ESCC progression via regulating multiple signal pathways.

Authors - Xiaofang Duan