RADIL Polyclonal antibody

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• KD/KO Validated

RADIL Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20284-1-AP

Synonyms

KIAA1849, RADIL

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Validation Data Gallery

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  WB analysis using 20284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20284-1-AP (RADIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20284-1-AP

  IP result of anti-RADIL (IP:20284-1-AP, 4ug; Detection:20284-1-AP 1:1000) with HEK-293 cells lysate 1400 ug.

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  IF Staining of HeLa using 20284-1-AP

  Immunofluorescent analysis of Hela cells, using RADIL antibody 20284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, SH-SY5Y cells, mouse testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

20284-1-AP targets RADIL in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RADIL fusion protein Ag13996
• Full Name: Ras association and DIL domains
• Calculated Molecular Weight: 1075 aa, 117 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC004919
• Gene Symbol: RADIL
• Gene ID (NCBI): 55698
• RRID: AB_10732684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Radil is a broadly expressed adapter protein that contains a RA-like domain, a PDZ domain and a DIL domain. Like RAPL and RIAM, Radil interacts preferentially with active GTP-bound Rap1, and overexpression of Radil enhances integrin-mediated adhesion. In addition, Radil knockdown inhibits cell-substrate adhesion in HT1080, AD293, and NMuMG cells and results in migration defects in SW1353 and zebrafish neural crest cells (PMID: 17704304). Radil regulates neutrophil adhesion by specifically enhancing β2-integrin activation, as well as focal adhesion kinase (FAK) and paxillin phosphorylation (PMID: 23097489).

Publications

Species	Application	Title
human	WB	Cancers (Basel); Proteomic Mapping of the Interactome of KRAS Mutants Identifies New Features of RAS Signalling Networks and the Mechanism of Action of Sotorasib; Authors - Aoife Nolan
human	WB	J Biol Chem; Identification of Radil as a Ras binding partner and putative activator.; Authors - Byeong Hyeok Choi ; KD Validated