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RAD52 Monoclonal antibody, PBS Only

RAD52 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H8G9

Cat no : 68283-1-PBS

Synonyms

RAD52, RAD52 homolog (S. cerevisiae)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD52 Monoclonal antibody (68283-1-Ig)

    WB analysis using 68283-1-Ig (same clone as 68283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68283-1-Ig (RAD52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68283-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68283-1-PBS targets RAD52 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAD52 fusion protein Ag27665
Full Name RAD52 homolog (S. cerevisiae)
Calculated Molecular Weight 46 aa
Observed Molecular Weight46 kDa
GenBank Accession NumberNM_001297419
Gene Symbol RAD52
Gene ID (NCBI) 5893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

In Saccharomyces cerevisiae, Rad52 functions as a critical mediator of homologous recombination (HR) by facilitating the loading of Rad51 onto ssDNA. However, in mammalian systems and other vertebrates, BRCA2 is known to function as the primary mediator of RAD51 filament formation, while prior studies have shown a limited role for RAD52 in HR.Additionally, loss of RAD52 has little consequence for cell viability. Recent evidence suggests that RAD52 may provide an alternative mediator pathway to BRCA2 function (PMID: 34928169). Several studies have suggested a link between the functional regulation of RAD52 and the protein modification by a small ubiquitin-like modifier (SUMO), sumoylation may play an important role in the nuclear transport of RAD52 (PMID:20190268). The calculated MW of Rad52 is 46 kDa, 68283-1-Ig can detect the band around 46 kDa.