RAD52 Polyclonal antibody

RAD52 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 55020-1-AP

Synonyms

RAD52, RAD52 homolog (S. cerevisiae)

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Validation Data Gallery

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  WB analysis of PC-3 using 55020-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55020-1-AP (RAD52 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 55020-1-AP

  IP result of anti-RAD52 (IP:55020-1-AP, 4ug; Detection:55020-1-AP 1:300) with PC-3 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IP detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55020-1-AP targets RAD52 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAD52 homolog (S. cerevisiae)
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: NM_134424
• Gene Symbol: RAD52
• Gene ID (NCBI): 5893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.