RAD51C Polyclonal antibody

RAD51C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31678-1-AP

Synonyms

R51H3, RAD51 homolog C, RAD51 like protein 2, RAD51C, RAD51L2

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Validation Data Gallery

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  WB analysis using 31678-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31678-1-AP (RAD51C antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, MDA-MB-231 cells, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31678-1-AP targets RAD51C in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD51C fusion protein Ag35070
• Full Name: RAD51 homolog C (S. cerevisiae)
• Calculated Molecular Weight: 376 aa, 42 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC107753
• Gene Symbol: RAD51C
• Gene ID (NCBI): 5889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).