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RAD51AP1 Monoclonal antibody, PBS Only

RAD51AP1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8B1

Cat no : 68560-1-PBS

Synonyms

PIR51, RAD51 associated protein 1, RAD51 interacting protein, RAD51AP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD51AP1 Monoclonal antibody (68560-1-Ig)

    WB analysis using 68560-1-Ig (same clone as 68560-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68560-1-Ig (RAD51AP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68560-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68560-1-PBS targets RAD51AP1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAD51AP1 fusion protein Ag28385
Full Name RAD51 associated protein 1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight38-40 kDa
GenBank Accession NumberBC016330
Gene Symbol RAD51AP1
Gene ID (NCBI) 10635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].