RAD51AP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAD51AP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ChIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 11255-1-AP

Synonyms

RAD51-interacting protein, RAD51-associated protein 1, RAD51 interacting protein, RAD51 associated protein 1, PIR51

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Validation Data Gallery

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  WB analysis using 11255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11255-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11255-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11255-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11255-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 11255-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11255-1-AP (RAD51AP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, mouse liver tissue, rat liver tissue, Raji cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Product Information

11255-1-AP targets RAD51AP1 in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD51AP1 fusion protein Ag1781
• Full Name: RAD51 associated protein 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC016330
• Gene Symbol: RAD51AP1
• Gene ID (NCBI): 10635
• RRID: AB_2300786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].

Publications

Species	Application	Title
human	WB,IF	Nature; RNA transcripts stimulate homologous recombination by forming DR-loops.; Authors - Jian Ouyang ; KD Validated
human	WB	Mol Cell; TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch; Authors - Tribhuwan Yadav
human	WB	Oncogene; BRD4 inhibition sensitizes cervical cancer to radiotherapy by attenuating DNA repair.; Authors - Mengdong Ni
human,mouse	IF	J Biol Chem; Pre-rRNA facilitates the recruitment of RAD51AP1 to DNA double-strand breaks; Authors - Linlin Chen
human	WB	Front Genet; Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis; Authors - Renwang Liu
mouse	WB	J Cell Biochem; The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.; Authors - Shuhei Ishikura