RAD51 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RAD51 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4A12B8

Cat no : 67024-1-PBS

Synonyms

BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA

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Validation Data Gallery

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  WB analysis using 67024-1-Ig (same clone as 67024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67024-1-Ig (same clone as 67024-1-PBS)

  WB result of RAD51 antibody (67024-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67024-1-Ig (same clone as 67024-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67024-1-Ig (same clone as 67024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67024-1-Ig (same clone as 67024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67024-1-PBS targets RAD51 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAD51 fusion protein Ag7410
• Full Name: RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001459
• Gene Symbol: RAD51
• Gene ID (NCBI): 5888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.