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  • KD/KO Validated

RAD51 Monoclonal antibody

RAD51 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human, mouse, rat and More (1)







Cat no : 67024-1-Ig


BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA

Tested Applications

Positive WB detected inJurkat cells, 4T1 cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67024-1-Ig targets RAD51 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAD51 fusion protein Ag7410
Full Name RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC001459
Gene Symbol RAD51
Gene ID (NCBI) 5888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.


Product Specific Protocols
WB protocol for RAD51 antibody 67024-1-IgDownload protocol
IHC protocol for RAD51 antibody 67024-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Juri (Verified Customer) (01-02-2024)

We have tested in immunocytochemistry, but did not work. Kindly, proteintech offered a replacement.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 in 200
  • Cell Tissue Type: Cell