RAD50 Polyclonal antibody

RAD50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29390-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

DNA repair protein RAD50, hRad50, RAD50, RAD50 2, RAD50 homolog (S. cerevisiae)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inC6 cells, Jurkat cells, K-562 cells, MCF-7 cells, MOLT-4 cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29390-1-AP targets RAD50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD50 fusion protein Ag29949 Predict reactive species
Full Name RAD50 homolog (S. cerevisiae)
Calculated Molecular Weight 154 kDa
Observed Molecular Weight154 kDa
GenBank Accession NumberNM_005732
Gene Symbol RAD50
Gene ID (NCBI) 10111
RRIDAB_2918289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92878
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAD50 antibody 29390-1-APDownload protocol
IHC protocol for RAD50 antibody 29390-1-APDownload protocol
IF protocol for RAD50 antibody 29390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Transl Med

Modulation of SRC by SNTB1 activates the Hippo-YAP pathway during colon adenocarcinoma metastasis

Authors - Zhengyan Chang
humanIF

Cell Genom

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors

Authors - Yahui Wang
humanWB

CNS Neurosci Ther

Deep-targeted gene sequencing reveals ARID1A mutation as an important driver of glioblastoma

Authors - Menglin Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Veronica (Verified Customer) (11-14-2022)

The antibody seems to work correctly. However, further tests need to be done.

  • Applications: Immunofluorescence
  • Cell Tissue Type: hTERT RPE-1
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