RAD23A Polyclonal antibody, PBS Only

RAD23A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51033-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of mouse spleen using 51033-1-AP (same clone as 51033-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 51033-1-AP (same clone as 51033-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 51033-1-AP (same clone as 51033-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 51033-1-AP (same clone as 51033-1-PBS)

  IP result of anti-RAD23A (IP:51033-1-AP, 4ug; Detection:51033-1-AP 1:500) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 51033-1-AP (same clone as 51033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 51033-1-AP (RAD23A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51033-1-PBS in a different storage buffer formulation.

---

Product Information

51033-1-PBS targets RAD23A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD23A fusion protein Ag0716
• Full Name: RAD23 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC014026
• Gene Symbol: RAD23A
• Gene ID (NCBI): 5886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RAD23A, also named as HHR23A, belongs to the RAD23 family. It is a multiubiquitin chain receptor involved in modulation of proteasomal degradation. RAD23A binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. It is proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. It is involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. It is also involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages and is required for the association of HIV-1 vpr with the host proteasome. This antibody is a rabbit polyclonal antibody raised against a fusion protein of full length human RAD23A.