RAD18 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAD18 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 18333-1-AP

Synonyms

RING-type E3 ubiquitin transferase RAD18, RING finger protein 73, hRAD18, hHR18, EC:2.3.2.27

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Validation Data Gallery

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  WB analysis of K-562 using 18333-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18333-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18333-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18333-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18333-1-AP

  IP result of anti-RAD18 (IP:18333-1-AP, 3ug; Detection:18333-1-AP 1:300) with K-562 cells lysate 2000ug.

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  IHC staining of human ovary cancer using 18333-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18333-1-AP (RAD18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 18333-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18333-1-AP (RAD18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, A549 cells, HeLa cells, MCF-7 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Product Information

18333-1-AP targets RAD18 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD18 fusion protein Ag12944
• Full Name: RAD18 homolog (S. cerevisiae)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 75 kDa, 85 kDa
• GenBank Accession Number: BC001302
• Gene Symbol: RAD18
• Gene ID (NCBI): 56852
• RRID: AB_2176586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively(PMID: 15509568). Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Regulation of the DNA damage response on male meiotic sex chromosomes.; Authors - Lu Lin-Yu LY
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	IF	Nucleic Acids Res; REV1 and polymerase ζ facilitate homologous recombination repair.; Authors - Sharma Shilpy S
human	WB	J Cell Sci; RNF168 forms a functional complex with RAD6 during the DNA damage response.; Authors - Liu Chao C
human	IF	Cell Cycle; A small ubiquitin binding domain inhibits ubiquitin-dependent protein recruitment to DNA repair foci.; Authors - Corey M Helchowski
human	WB,IF	Mol Pharmacol; DNA polymerase ζ is a major determinant of resistance to platinum-based chemotherapeutic agents.; Authors - Sharma Shilpy S

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandra (Verified Customer) (03-20-2024)

The middle part of the picture is irrelevant (other sample, other antibody), but I did not want to modify the picture. The antibody works perfect, on the picture the endogenous Rad18 can be seen. It works fine for the endogenous protein visualisation in 1000x dilution, incubation ON.

• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS human osteosarcoma cell line

RAD18 Antibody Western Blot,Immunohistochemistry,Immunoprecipitation,Cell culture validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:18333-1-AP)