RACGAP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

RACGAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6E10A2

Cat no : 66056-1-Ig

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

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Validation Data Gallery

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  WB analysis of HEK-293 using 66056-1-Ig

  HEK-293, Jurkat, ROS1728, PC-12, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK293 cells using 66056-1-Ig

  WB result of RACGAP1 antibody (66056-1-Ig, 1:3000) with si-control and si-RACGAP1 transfected HEK293 cells.

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  WB analysis of human brain using 66056-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66056-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66056-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66056-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66056-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66056-1-Ig

  Immunofluorescent analysis of HeLa cells, using RACGAP1 antibody 66056-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HEK293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66056-1-Ig is RACGAP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RACGAP1 fusion protein Ag6944
• Full Name: Rac GTPase activating protein 1
• Calculated Molecular Weight: 632 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC032754
• Gene Symbol: RACGAP1
• Gene ID (NCBI): 29127
• RRID: AB_11043178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.