RACGAP1 Polyclonal antibody

RACGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30042-1-AP

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30042-1-AP targets RACGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RACGAP1 fusion protein Ag32468
Full Name Rac GTPase activating protein 1
Calculated Molecular Weight 632 aa, 71 kDa
Observed Molecular Weight71 kDa
GenBank Accession NumberBC032754
Gene Symbol RACGAP1
Gene ID (NCBI) 29127
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RACGAP1 antibody 30042-1-APDownload protocol
IHC protocol for RACGAP1 antibody 30042-1-APDownload protocol
IF protocol for RACGAP1 antibody 30042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols