RACGAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RACGAP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 13739-1-AP

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

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Validation Data Gallery

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  WB analysis of K-562 using 13739-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 13739-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13739-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13739-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13739-1-AP

  IP result of anti-RACGAP1 (IP:13739-1-AP, 4ug; Detection:13739-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IP experiment of HeLa cells using 13739-1-AP

  IP result of anti-RACGAP1 (13739-1-AP for IP and Detection).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, RAW 264.7 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Product Information

13739-1-AP targets RACGAP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RACGAP1 fusion protein Ag4676
• Full Name: Rac GTPase activating protein 1
• Calculated Molecular Weight: 632 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC032754
• Gene Symbol: RACGAP1
• Gene ID (NCBI): 29127
• RRID: AB_2176276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

Publications

Species	Application	Title
human	WB,IHC	Front Cell Dev Biol; PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells.; Authors - Shixin Liao
mouse	WB,IHC	Front Pharmacol; Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway.; Authors - Fujing Wang
mouse	IF	Mol Biol Cell; Chromokinesin Kif4 promotes proper anaphase in mouse oocyte meiosis.; Authors - Carissa M Heath
human	WB,IHC	Cancers (Basel); Lambda-Carrageenan Enhances the Effects of Radiation Therapy in Cancer Treatment by Suppressing Cancer Cell Invasion and Metastasis through Racgap1 Inhibition.; Authors - Ping-Hsiu Wu
human	WB	Cell Death Dis; Pseudogene RACGAP1P activates RACGAP1/Rho/ERK signalling axis as a competing endogenous RNA to promote hepatocellular carcinoma early recurrence.; Authors - Meng-Yao Wang
human	WB,IHC,IF	Cell Death Dis; PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling; Authors - Hualin Chen