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  • KD/KO Validated

RABL3 Polyclonal antibody

RABL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 11916-1-AP

Synonyms

Rab-like protein 3, Rab like protein 3



Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, human brain tissue, human lung tissue, HUVEC cells, U-251 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human cervical cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11916-1-AP targets RABL3 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABL3 fusion protein Ag2531
Full Name RAB, member of RAS oncogene family-like 3
Calculated Molecular Weight 236 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC020832
Gene Symbol RABL3
Gene ID (NCBI) 285282
RRIDAB_2175960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

Protocols

Product Specific Protocols
WB protocol for RABL3 antibody 11916-1-APDownload protocol
IHC protocol for RABL3 antibody 11916-1-APDownload protocol
IF protocol for RABL3 antibody 11916-1-APDownload protocol
IP protocol for RABL3 antibody 11916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells.

Authors - Li Qi Q
  • KD Validated
humanWB

Med Sci Monit

High Expression of Rab-like 3 (Rabl3) is Associated with Poor Survival of Patients with Non-Small Cell Lung Cancer via Repression of MAPK8/9/10-Mediated Autophagy.

Authors - Weihua Zhang
  • KD Validated