RABIF Polyclonal antibody, PBS Only

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  WB analysis using 29113-1-AP (same clone as 29113-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29113-1-AP (RABIF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 29113-1-AP (same clone as 29113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 29113-1-AP (same clone as 29113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 29113-1-AP (same clone as 29113-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABIF antibody (29113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

RABIF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29113-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

29113-1-PBS targets RABIF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RABIF fusion protein Ag30761
• Full Name: RAB interacting factor
• Calculated Molecular Weight: 123 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC018488
• Gene Symbol: RABIF
• Gene ID (NCBI): 5877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P47224
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) was the first putative guanine nucleotide exchange factors (GEFs) isolated for Rabs, which may act as a chaperone, allowing interacting Rab proteins to be properly activated where and when they are needed in the cell (PMID: 25158853). RABIF is a 17-kDa protein that is expressed in all mammalian tissues and is in complex with nucleotide-free Rab8 GTPase and Rab10 GTPase (PMID: 28894007, 28228250).