RabGEF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RabGEF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12735-1-AP

Synonyms

RAP1, Rabex-5, RABEX5, Rabaptin-5-associated exchange factor for Rab5, Rab5 GDP/GTP exchange factor

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Validation Data Gallery

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  WB analysis of PC-3 using 12735-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12735-1-AP (RABGEF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 12735-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12735-1-AP (RABGEF1 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of PC-3 using 12735-1-AP

  Immunofluorescent analysis of PC-3 cells using 12735-1-AP (RabGEF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

12735-1-AP targets RabGEF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RabGEF1 fusion protein Ag3442
• Full Name: RAB guanine nucleotide exchange factor (GEF) 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC015330
• Gene Symbol: RABGEF1
• Gene ID (NCBI): 27342
• RRID: AB_2175788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RabGEF1 (Rabex-5) is a guanine nucleotide exchange factor (GEF) for the endocytic pathway regulator Rab5. RabGEF1 contains a Vps9 domain, an A20-like zinc finger (ZnF) domain, and a coiled coil domain (PMID: 17341663). RabGEF1 is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It is also implicated in tumorigenesis and in the development of certain human cancers (PMID: 25427001).

Publications

Species	Application	Title
mouse	IF	Sci Rep; AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway.; Authors - Ermes Candiello
human	WB	Exp Mol Pathol; RabGEF1 functions as an oncogene in U251 glioblastoma cells and is involved in regulating AKT and Erk pathways.; Authors - Haitao Fan ; KD Validated
human,rat	WB	Orthop Surg; LncRNA TM1-3P Regulates Proliferation, Apoptosis and Inflammation of Fibroblasts in Osteoarthritis through miR-144-3p/ONECUT2 Axis; Authors - Yangfei Yi
mouse	WB	Cell Rep; Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease.; Authors -
human	WB	J Cell Sci; Vps9d1 regulates tubular endosome formation through specific activation of Rab22A; Authors - Shumpei Nakashima