RABGAP1L Recombinant antibody

RABGAP1L Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240663G12

Cat no : 83698-4-RR

Synonyms

Rab GTPase-activating protein 1-like, isoform 10, 240663G12

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Validation Data Gallery

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  WB analysis using 83698-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83698-4-RR (RABGAP1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83698-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RABGAP1L Recombinant antibody (83698-4-RR, Clone:240663G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83698-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83698-4-RR against Human RABGAP1L were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83698-4-RR is RABGAP1L Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RABGAP1L fusion protein Ag4856
• Full Name: RAB GTPase activating protein 1-like
• Calculated Molecular Weight: 815 aa, 93 kDa
• Observed Molecular Weight: 100 kDa,140 kDa
• GenBank Accession Number: BC041888
• Gene Symbol: RABGAP1L
• Gene ID (NCBI): 9910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain.