RABGAP1L Polyclonal antibody

• Featured Product
• KD/KO Validated

RABGAP1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13894-1-AP

Synonyms

Rab GTPase-activating protein 1-like, isoform 10

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Validation Data Gallery

• 

  WB analysis of HeLa using 13894-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13894-1-AP (RABGAP1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13894-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13894-1-AP (RABGAP1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13894-1-AP

  IP result of anti-RABGAP1L (IP:13894-1-AP, 4ug; Detection:13894-1-AP 1:1000) with HeLa cells lysate 3200ug.

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  IHC staining of human hepatocirrhosis using 13894-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 13894-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 13894-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 13894-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 13894-1-AP

  Immunofluorescent analysis of Hela cells, using RABGAP1L antibody 13894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human hepatocirrhosis tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13894-1-AP targets RABGAP1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RABGAP1L fusion protein Ag4856
• Full Name: RAB GTPase activating protein 1-like
• Calculated Molecular Weight: 815 aa, 93 kDa
• Observed Molecular Weight: 93-105 kDa
• GenBank Accession Number: BC041888
• Gene Symbol: RABGAP1L
• Gene ID (NCBI): 9910
• RRID: AB_10638308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain.

Publications

Species	Application	Title
human	IHC	Nat Commun; The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer.; Authors - Emily Golden
human	WB,IF	Cell Rep; Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.; Authors - Sonja Fernbach
mouse	WB	J Cell Biol; TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1; Authors - Shu Hiragi ; KD Validated
rat	IHC	BMC Pregnancy Childbirth; Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy; Authors - Yan Fang