RABEP2 Polyclonal antibody

RABEP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 14625-1-AP

Synonyms

RABPT5B, RABEP 2, Rabaptin-5beta, Rab GTPase-binding effector protein 2, FRA

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Validation Data Gallery

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  WB analysis of Jurkat using 14625-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14625-1-AP (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14625-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14625-1-AP (RABEP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14625-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14625-1-AP (RABEP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14625-1-AP

  IP result of anti-RABEP2 (IP:14625-1-AP, 4ug; Detection:14625-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14625-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABEP2 antibody (14625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human cervical cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 2 publications below

Product Information

14625-1-AP targets RABEP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RABEP2 fusion protein Ag6197
• Full Name: rabaptin, RAB GTPase binding effector protein 2
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 59-62 kDa
• GenBank Accession Number: BC058900
• Gene Symbol: RABEP2
• Gene ID (NCBI): 79874
• RRID: AB_2175655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.

Publications

Species	Application	Title
human	WB	Am J Hum Genet; A cross-species approach using an in vivo evaluation platform in mice demonstrates that sequence variation in human RABEP2 modulates ischemic stroke outcomes; Authors - Han Kyu Lee
mouse	WB	Stroke; Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke.; Authors - Jennifer L Lucitti
human	WB,IF	Sci Rep; Rab-GTPase binding effector protein 2 (RABEP2) is a primed substrate for Glycogen Synthase kinase-3 (GSK3).; Authors - Lisa Logie
mouse	WB	J Mol Cell Cardiol; Decreased inspired oxygen stimulates de novo formation of coronary collaterals in adult heart.; Authors - Amir Aghajanian
mouse	WB	J Hematol Oncol; LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner.; Authors - Wen-Tao Wang
mouse	WB,IF	Arterioscler Thromb Vasc Biol; Hypoxia-Preconditioned Bone Marrow Mesenchymal Stem Cells Improved Cerebral Collateral Circulation and Stroke Outcome in Mice; Authors - Hao Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (04-22-2024)

Excellent antibody for detection of RABEP2 by WB or IF.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1 in 1000 WB and 1 in 200 IF
• Cell Tissue Type: HUVEC