RAB9A Recombinant antibody

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  WB analysis using 84129-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84129-5-RR (RAB9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of U2OS using 84129-5-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug RAB9A Recombinant antibody (84129-5-RR, Clone:241372F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84129-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84129-5-RR against Human RAB9A were performed. The affinity constant is 11.9 nM.

RAB9A {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84129-5-RR

Clone No.241372F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

RAB9, Ras-related protein Rab-9A, Ras related protein Rab 9A, 241372F8

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Tested Applications

• Positive WB detected in: Jurkat cells, NIH/3T3 cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84129-5-RR targets RAB9A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAB9A fusion protein Ag1969
• Full Name: RAB9A, member RAS oncogene family
• Calculated Molecular Weight: 201 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC017265
• Gene Symbol: RAB9A
• Gene ID (NCBI): 9367
• RRID: AB_3671688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P51151
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.