RAB8B Polyclonal antibody, PBS Only

RAB8B Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55295-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse skin using 55295-1-AP (same clone as 55295-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55295-1-AP (same clone as 55295-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 55295-1-AP (same clone as 55295-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 55295-1-AP (same clone as 55295-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 55295-1-AP (same clone as 55295-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55295-1-PBS in a different storage buffer formulation.

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Product Information

55295-1-PBS targets RAB8B in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: RAB8B, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: NM_016530
• Gene Symbol: RAB8B
• Gene ID (NCBI): 51762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.