RAB6B Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB6B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 10340-1-AP

Synonyms

Ras-related protein Rab-6B, Ras related protein Rab 6B, EC:3.6.5.2

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Validation Data Gallery

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  WB analysis of mouse brain using 10340-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10340-1-AP (RAB6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10340-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10340-1-AP (RAB6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10340-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10340-1-AP (RAB6B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10340-1-AP

  IP result of anti-RAB6B (IP:10340-1-AP, 3ug; Detection:10340-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 10340-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10340-1-AP (RAB6B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10340-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10340-1-AP (RAB6B antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of C6 using 10340-1-AP

  Immunofluorescent analysis of C6 cells using 10340-1-AP (RAB6B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, C6 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

10340-1-AP targets RAB6B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB6B fusion protein Ag0322
• Full Name: RAB6B, member RAS oncogene family
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC002510
• Gene Symbol: RAB6B
• Gene ID (NCBI): 51560
• RRID: AB_2175461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human RAB genes share structural and biochemical properties with the Ras gene superfamily. Accumulating data suggests an important role for RAB proteins either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from endoplasmic reticulum to the Golgi complex and to secretory vesicles involves the movement of carrier vesicles, a process that appears to involve RAB protein function. Rab6A has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Rab6B is encoded by an independent gene which is located on chromosome 3 region q21-q23. In contrast to Rab6A whose expression is ubiquitous, Rab6B is expressed in a tissue and cell-type specific manner. Rab6B is predominantly expressed in brain and the neuroblastoma cells. In brain, Rab6B was found to be specifically expressed in microglia, pericytes and Purkinje cells. Endogenous Rab6B localises to the Golgi apparatus and to ERGIC-53-positive vesicles. Comparable studies between Rab6A and Rab6B revealed distinct biochemical and cellular properties. Rab6B displays lower GTP-binding activities and is distributed over Golgi and ER membranes, whereas Rab6A is more restricted to the Golgi apparatus. Since the GTP-bound form of Rab6B does interact with all known Rab6A effectors, including Rabkinesin-6, the results suggest a cell-type specific role for Rab6B in retrograde membrane traffic at the level of the Golgi complex.

Publications

Species	Application	Title
rat	IF	J Neuroimmunol; Autoantibody against the Rab6A/Rab6B in primary autoimmune cerebellar ataxia associated with Sjogren's syndrome: A case report.; Authors - Liyuan Guo
mouse	WB	Proteome Sci; PMID: 29760588; Authors - Elisa Ghelfi
mouse	WB	EMBO Rep; RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination; Authors - Sabine Bardin ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sahil (Verified Customer) (09-02-2020)

Works great as expected.

• Applications: Western Blot