Various lysates were subjected to SDS PAGE followed by western blot with 83949-4-RR (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83949-4-RR (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 83949-4-RR
Biolayer interferometry (BLl) kinetic assays of 83949-4-RR
against Human RAB5A were performed. The affinity constant is 26.7 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.
Various lysates were subjected to SDS PAGE followed by western blot with 83949-4-RR (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 83949-4-RR
Biolayer interferometry (BLl) kinetic assays of 83949-4-RR
against Human RAB5A were performed. The affinity constant is 26.7 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RAB5A Recombinant antibody
Catalog Number
83949-4-RR
Citations
-
Dilutions
WB : 1:5000-1:50000
Applications
WB, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
