RAB5A Recombinant antibody

RAB5A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241041D7

Cat no : 83949-4-RR

Synonyms

RAB5, Ras-related protein Rab-5A, Ras related protein Rab 5A, EC:3.6.5.2, 241041D7

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Validation Data Gallery

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  WB analysis using 83949-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83949-4-RR (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83949-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83949-4-RR against Human RAB5A were performed. The affinity constant is 26.7 nM.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tisue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83949-4-RR is RAB5A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAB5A fusion protein Ag2549
• Full Name: RAB5A, member RAS oncogene family
• Calculated Molecular Weight: 215 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC001267
• Gene Symbol: RAB5A
• Gene ID (NCBI): 5868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.