RAB43 Monoclonal antibody

RAB43 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B2A8

Cat no : 66654-1-Ig

Synonyms

ISY1, RAB41, RAB43, Ras related protein Rab 41, Ras related protein Rab 43



Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, HepG2 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66654-1-Ig targets RAB43 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB43 fusion protein Ag26911
Full Name RAB43, member RAS oncogene family
Calculated Molecular Weight 212 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC062319
Gene Symbol RAB43
Gene ID (NCBI) 339122
RRIDAB_2882011
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ras-related GTP-binding protein 43 (RAB43) is a member of the Ras superfamily. RAB43 is mainly located in endoplasmic reticulum and Golgi, and plays a role as a key regulator of vesicle movement, signal transduction and tethering membrane events in membrane trafficking.

Protocols

Product Specific Protocols
WB protocol for RAB43 antibody 66654-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

J Cell Sci

ARF6 plays a general role in targeting palmitoylated proteins from the Golgi to the plasma membrane

Authors - Juan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tsimafei (Verified Customer) (11-19-2024)

Great antibody for Rab43 detection

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U2OS
RAB43 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:66654-1-Ig)
FH

Moritz (Verified Customer) (09-12-2024)

Several bands were observable in WB. HEK293T Wt lysate.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
RAB43 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:66654-1-Ig)