RAB3IP/Rabin8 Polyclonal antibody

RAB3IP/Rabin8 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 29818-1-AP

Synonyms

RAB3IP, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein

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Validation Data Gallery

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  WB analysis using 29818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29818-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 29818-1-AP

  IP result of anti-RAB3IP/Rabin8 (IP:29818-1-AP, 4ug; Detection:29818-1-AP 1:4000) with Jurkat cells lysate 1320 ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29818-1-AP targets RAB3IP/Rabin8 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB3IP/Rabin8 fusion protein Ag31291
• Full Name: RAB3A interacting protein (rabin3)
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC015548
• Gene Symbol: RAB3IP
• Gene ID (NCBI): 117177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles.