RAB3GAP1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RAB3GAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A3A1

Cat no : 66688-1-PBS

Synonyms

DKFZp434A012, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1

---

Validation Data Gallery

• 

  WB analysis using 66688-1-Ig (same clone as 66688-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66688-1-Ig (same clone as 66688-1-PBS)

  WB result of RAB3GAP1 antibody (66688-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB3GAP1 transfected HeLa cells. This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66688-1-Ig (RAB3GAP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 66688-1-Ig (same clone as 66688-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAB3GAP1 antibody (66688-1-Ig, Clone: 4A3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66688-1-PBS in a different storage buffer formulation.

---

Product Information

66688-1-PBS targets RAB3GAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB3GAP1 fusion protein Ag17710
• Full Name: RAB3 GTPase activating protein subunit 1 (catalytic)
• Calculated Molecular Weight: 981 aa, 111 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC146809
• Gene Symbol: RAB3GAP1
• Gene ID (NCBI): 22930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.