RAB3D Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB3D Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (3)

Cat no : 12320-1-AP

Synonyms

D2 2, GOV, RAB16, RAB3D, RAD3D, Ras related protein Rab 3D

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Validation Data Gallery

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  WB analysis of mouse lung using 12320-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12320-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW 1990 using 12320-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 12320-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 12320-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 12320-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12320-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 12320-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 12320-1-AP (RAB3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SW 1990 using 12320-1-AP

  IP result of anti-RAB3D (IP:12320-1-AP, 3ug; Detection:12320-1-AP 1:600) with SW 1990 cells lysate 2400ug.

• 

  IHC staining of human prostate cancer using 12320-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12320-1-AP (RAB3D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12320-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12320-1-AP (RAB3D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12320-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12320-1-AP (RAB3D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12320-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12320-1-AP (RAB3D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, A549 cells, human cerebellum tissue, human stomach tissue, mouse spleen tissue, mouse stomach tissue, SW 1990 cells
• Positive IP detected in: SW 1990 cells
• Positive IHC detected in: human prostate cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 9 publications below
• IF: See 4 publications below

Product Information

12320-1-AP targets RAB3D in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB3D fusion protein Ag2973
• Full Name: RAB3D, member RAS oncogene family
• Calculated Molecular Weight: 219 aa, 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC016471
• Gene Symbol: RAB3D
• Gene ID (NCBI): 9545
• RRID: AB_2177228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab3D is a member of the Rab3 subfamily, which comprises four isoforms (Rab3A, Rab3B, Rab3C, and Rab3D). Rab3 proteins typically associate with secretory vesicles, including synaptic vesicles, and have been implicated in the control of regulated exocytosis. Rab3D is widely expressed in adipocytes, exocrine glands and several haematopoietic cells, where it localizes to secretory granules and vesicles.

Publications

Species	Application	Title
pig	WB	Mol Metab; Maternal hyperglycemia induces alterations in hepatic amino acid, glucose and lipid metabolism of neonatal offspring: Multi-omics insights from a diabetic pig model; Authors - Bachuki Shashikadze
		Sci Signal; RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms.; Authors - Junning Yang
human	IHC	Mol Cancer; Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.; Authors - Yu-Chan Chang
human	IHC	PLoS One; Accumulation of secretory vesicles in the lacrimal gland epithelia is related to non-Sjögren's type dry eye in visual display terminal users.; Authors - Mizuka Kamoi
mouse	IF	Am J Physiol Gastrointest Liver Physiol; Establishment of novel in vitro mouse chief cell and SPEM cultures identifies MAL2 as a marker of metaplasia in the stomach.; Authors - Victoria G Weis
human	WB	Cancer Manag Res; The lncRNA HOXA11-AS regulates Rab3D expression by sponging miR-125a-5p promoting metastasis of osteosarcoma.; Authors - Kun Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Rab3D antibody showed strong bands on western blot with fewer non-specific bands. This antibody was dilutedn1:2000 in 1X TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue

FH

robert (Verified Customer) (06-13-2022)

This Rab3D antibody worked well for both immunoblotting and immunocytochemistry with gastric cancer cell lines. I tried others from other companies, but this antibody worked best.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cell culture mkn45 and AGS cells

FH

Robert (Verified Customer) (01-24-2019)

This Rab3D antibody delivered a strong signal on Western Blots and fewer non-specific bands than other commercial Rab3D antibodies I've used. It was sensitive and specific. I may increase the dilution to 1:2000 and see how that works.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Gastric cell lines, pancreas