RAB37 Recombinant antibody

RAB37 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240838D6

Cat no : 83740-4-RR

Synonyms

Ras-related protein Rab-37, Ras related protein Rab 37, 240838D6

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Validation Data Gallery

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  WB analysis using 83740-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83740-4-RR (RAB37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83740-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83740-4-RR against Human RAB37 were performed. The affinity constant is 1.25 nM.

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells, mouse brain tissues, rat brain tissues

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83740-4-RR is RAB37 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAB37 fusion protein Ag3717
• Full Name: RAB37, member RAS oncogene family
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016615
• Gene Symbol: RAB37
• Gene ID (NCBI): 326624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB37, as a small GTPase, plays important roles in several cellular processes through intracellular membrane traffic. RAB37 can regulate insulin secretion in β-cells 7 and TNF-α secretion from activated macrophages. In allergic responses, RAB37 regulated mast cell degranulation. RAB37 has been identified to be a tumor suppressor and it regulated exocytosis of several proteins including TIMP metallopeptidase inhibitor 1 (TIMP1), secreted frizzled-related protein-1 (SFRP1) 12, thrombospondin-1 (TSP1), and soluble ST2.