• Featured Product
  • KD/KO Validated

RAB35 Polyclonal antibody

RAB35 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 11329-2-AP


GTP binding protein RAY, H ray, RAB1C, RAB35, Ras related protein Rab 1C, Ras related protein Rab 35, RAY

Tested Applications

Positive WB detected inA431 cells, A375 cells, human brain tissue, HeLa cells, MCF-7 cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-87 MG cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11329-2-AP targets RAB35 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB35 fusion protein Ag1872
Full Name RAB35, member RAS oncogene family
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC015931
Gene Symbol RAB35
Gene ID (NCBI) 11021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.


Product Specific Protocols
WB protocol for RAB35 antibody 11329-2-APDownload protocol
IHC protocol for RAB35 antibody 11329-2-APDownload protocol
IF protocol for RAB35 antibody 11329-2-APDownload protocol
IP protocol for RAB35 antibody 11329-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Identification of an oncogenic RAB protein.

Authors - Douglas B Wheeler
  • KD Validated

Cell Metab

Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.

Authors - Chaitali Mukherjee

Nat Cell Biol

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Authors - James W Clancy

Nat Cell Biol

Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.

Authors - Alexander Wallroth

Sci Adv

LRRK2 mediates tubulation and vesicle sorting from lysosomes.

Authors - Luis Bonet-Ponce

Nat Commun

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Authors - Corallino Salvatore
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Xin (Verified Customer) (03-15-2022)

A clean antibody to probe Rab35 with the right MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human postmortem brain lysate
RAB35 Antibody Western Blot validation (1:2000 dilution) in human postmortem brain lysate (Cat no:11329-2-AP)

Tom (Verified Customer) (02-24-2021)

HEK293T whole cell lysate probed with Rab35 antibody overnight at 4 degrees. Membrane was blocked in 5% BSA for one hour prior to incubation with antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
RAB35 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11329-2-AP)

Mayur (Verified Customer) (01-14-2019)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain tissue (Cortex)
RAB35 Antibody Western Blot, validation (1:1000 dilution) in Mouse brain tissue (Cortex) (Cat no:11329-2-AP)