Validation Data Gallery
|Positive WB detected in||A431 cells, A375 cells, human brain tissue, HeLa cells, MCF-7 cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue, human liver tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11329-2-AP targets RAB35 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB35 fusion protein Ag1872|
|Full Name||RAB35, member RAS oncogene family|
|Calculated molecular weight||201 aa, 23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC015931|
|Gene ID (NCBI)||11021|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.
Identification of an oncogenic RAB protein.
Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.
Nat Cell Biol
An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.
Nat Cell Biol
Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.
LRRK2 mediates tubulation and vesicle sorting from lysosomes.
A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Xin (Verified Customer) (03-15-2022)
A clean antibody to probe Rab35 with the right MW.
Tom (Verified Customer) (02-24-2021)
HEK293T whole cell lysate probed with Rab35 antibody overnight at 4 degrees. Membrane was blocked in 5% BSA for one hour prior to incubation with antibody.
Mayur (Verified Customer) (01-14-2019)