RAB34 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB34 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27435-1-AP

Synonyms

Ras-related protein Rab-34, Ras related protein Rah, Ras related protein Rab 39, Ras related protein Rab 34, RAH

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Validation Data Gallery

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  WB analysis using 27435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27435-1-AP (RAB34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27435-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27435-1-AP (RAB34 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27435-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27435-1-AP (RAB34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, U2OS cells, A549 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 5 publications below

Product Information

27435-1-AP targets RAB34 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB34 fusion protein Ag26696
• Full Name: RAB34, member RAS oncogene family
• Calculated Molecular Weight: 251 aa, 28 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC016841
• Gene Symbol: RAB34
• Gene ID (NCBI): 83871
• RRID: AB_2880870
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB34, a Rab protein family member, is involved in repositioning of lysosomes, activation of micropinocytosis, and protein transport. RAB34 is aberrantly expressed in various cancers and exhibits oncogenic properties. Recently, Rab34 is identified as a ciliary protein. Rab34 is required for internal assembly of cilia but not for cilia built on the cell surface.

Publications

Species	Application	Title
mouse	IF, WB	Curr Biol; Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.; Authors - Anil Kumar Ganga ; KD Validated
human	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human	WB,IF	Biomedicines; Signaling Switching from Hedgehog-GLI to MAPK Signaling Potentially Serves as a Compensatory Mechanism in Melanoma Cell Lines Resistant to GANT-61; Authors - Nikolina Piteša
human	WB	Hum Mol Genet; Pathogenic RAB34 variants impair primary cilium assembly and cause a novel oral-facial-digital syndrome; Authors - Ange-Line Bruel
mouse	IF	bioRxiv; A hierarchical pathway for assembly of the distal appendages that organize primary cilia; Authors - Tomoharu Kanie
mouse	IF	bioRxiv; Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages; Authors - Tomoharu Kanie