RAB26 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB26 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14284-1-AP

Synonyms

Ras-related protein Rab-26, Ras related protein Rab 26

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Validation Data Gallery

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  WB analysis of mouse pancreas using 14284-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14284-1-AP (RAB26 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14284-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14284-1-AP (RAB26 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 14284-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14284-1-AP (RAB26 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 14284-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14284-1-AP (RAB26 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of BxPC-3 using 14284-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 14284-1-AP (RAB26 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 14284-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAB26 antibody (14284-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 14284-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RAB26 (14284-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, BxPC-3 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

14284-1-AP targets RAB26 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB26 fusion protein Ag5581
• Full Name: RAB26, member RAS oncogene family
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC066913
• Gene Symbol: RAB26
• Gene ID (NCBI): 25837
• RRID: AB_2176709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Sci Total Environ; Manganese-induced α-synuclein overexpression promotes the accumulation of dysfunctional synaptic vesicles and hippocampal synaptotoxicity by suppressing Rab26-dependent autophagy in presynaptic neurons; Authors - Zhuo Ma
human	WB,IF	J Cell Sci; RAB26 coordinates lysosome traffic and mitochondrial localization.; Authors - Ramon U Jin
human	IHC	Cell Death Dis; SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.; Authors - Nianli Liu ; KO Validated
human	WB, IHC	Cell Death Dis; Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src.; Authors - Huiying Liu
human	WB	J Immunol Res; Novel Circulating Tumour Cell-Related Risk Model Indicates Prognosis and Immune Infiltration in Lung Adenocarcinoma.; Authors - Lu Liang ; KD Validated
human,mouse	WB,IHC	Free Radic Biol Med; ROS induced the Rab26 promoter hypermethylation to promote cigarette smoking-induced airway epithelial inflammation of COPD through activation of MAPK signaling; Authors - Bin-Feng He ; KD Validated